Release of free fatty acids from Ehrlich ascites tumor cells.

The importance of free fatty acid in tumor nutrition.

Free fatty acid was found in the ascites fluid of the Ehrlich ascites tumor, about 3C¿ of the total fatty acid contained in the fluid being in unesterified form. The major components of the ascites free fatty acid fraction—palmitate, stéarate,oleate, and linoleate—also were the main species of fatty acid contained in the tumor cells. Tracer experiments with palmitate and oleate indicated ...

Short-term effects of free fatty acids on the regulation of fatty acid biosynthesis in Ehrlich ascites tumor cells.

Effect of palmitate, acetate and glucose on glutamine metabolism in Ehrlich ascites tumor cells.

Acetate and the long chain free fatty acid palmitate provoked a decrease in the rates of glutamine utilization and glutamate production in Ehrlich ascites tumor cells incubated with 0.5 mM glutamine. There was a cumulative effect with glucose on glutamine metabolism.

Effect of polylysine on the leakage and retention of compounds by Ehrlich ascites tumor cells.

Washed Ehrlich ascites tumor cells were suspended in either a saline or a polylysine saline solution. The cells were centrifuged, and the supernatant above the polylysinet reated or control cells or the cell pellet was studied. Inorganic phosphate, carbohydrate, free amino acids, small peptides, potassium, and adenosine-5'-monophosphate were released from the tumor cells into the supernatant af...

Effects of some fatty acid esters on the viability and transplantability of Ehrlich ascites tumor cells.

Fatty acids, monoglycerides, and some esters of fatty acids show antitumor activity against Ehrlich ascites tumor in mice. For investigation of the in vivo mode of action of these lipids, the effects of those fatty acid esters on animal cells in vitro were studied in sheep red blood cells and Ehrlich ascites tumor cells. Throughout these studies, we used fatty acid sucrose esters because they a...